6D0Q

Structure of a DNA retention-prone PCNA variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2017-03-31
Detector	RIGAKU SATURN 944
Wavelength(s)	1.54178
Spacegroup name	P 21 3
Unit cell lengths	122.264, 122.264, 122.264
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.910 - 2.801
R-factor	0.243303038576
Rwork	0.242
R-free	0.26913
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4yhr
RMSD bond length	0.006
RMSD bond angle	0.950
Data reduction software	HKL-3000
Data scaling software	HKL-3000 (v703x)
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.800	2.800
Rmerge	0.093	1.154
Rmeas	0.098	1.208
Rpim	0.029	0.354
Number of reflections	15284	757
<I/σ(I)>	27.54	1.65
Completeness [%]	99.9	100
Redundancy	11.5	11.5
CC(1/2)	0.996	0.842

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	298	50 mM sodium citrate pH 5.6, 2 M (NH4)2SO4