6D0M
Polymerase Eta post-insertion binary complex with cytarabine (AraC)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-10-24 |
Detector | DECTRIS PILATUS3 R CdTe 300K |
Wavelength(s) | 1.10000 |
Spacegroup name | P 61 |
Unit cell lengths | 98.566, 98.566, 81.477 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.680 - 1.832 |
R-factor | 0.1621 |
Rwork | 0.160 |
R-free | 0.19620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4j9p |
RMSD bond length | 0.006 |
RMSD bond angle | 0.825 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 1.860 |
High resolution limit [Å] | 1.830 | 1.830 |
Rmerge | 0.074 | 0.612 |
Number of reflections | 39467 | 1849 |
<I/σ(I)> | 31.4 | 2.25 |
Completeness [%] | 99.7 | 95.7 |
Redundancy | 12 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | MES buffer, PEG1500 |