6D0L
Structure of human TIRR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2016-10-11 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 |
Unit cell lengths | 41.890, 46.360, 48.800 |
Unit cell angles | 92.31, 108.07, 105.75 |
Refinement procedure
Resolution | 38.000 - 1.970 |
R-factor | 0.176 |
Rwork | 0.174 |
R-free | 0.20910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kvh |
RMSD bond length | 0.006 |
RMSD bond angle | 0.710 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.000 | 2.041 |
High resolution limit [Å] | 1.970 | 1.970 |
Rmerge | 0.084 | 0.338 |
Rmeas | 0.098 | 0.396 |
Number of reflections | 22070 | 1980 |
<I/σ(I)> | 15.12 | 5.15 |
Completeness [%] | 93.7 | 84.07 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.06 M citric acid, 0.04 M Bis-Tris-Propane, pH 4.1, 16% PEG 3,350; Cryoprotection in 25% PEG 3,350 |