6D0C

Crystal structure of HIF2a-B*:ARNT-B* complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97901
Spacegroup name	C 1 2 1
Unit cell lengths	73.604, 82.829, 41.321
Unit cell angles	90.00, 106.64, 90.00

Refinement procedure

Resolution	24.880 - 1.500
R-factor	0.2084
Rwork	0.207
R-free	0.24040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xt2
RMSD bond length	0.024
RMSD bond angle	2.390
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.550
High resolution limit [Å]	1.500	1.500
Rmeas	0.072	0.947
Rpim	0.038	0.555
Number of reflections	37270	3317
<I/σ(I)>	29.3	1.1
Completeness [%]	98.0	88.3
Redundancy	3.5	2.4
CC(1/2)		0.482

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.4	293	Bis-Tris, pH5.4 and 16% PEG3350, Seeding with crushed crystals right after setup