6D0C
Crystal structure of HIF2a-B*:ARNT-B* complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97901 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 73.604, 82.829, 41.321 |
| Unit cell angles | 90.00, 106.64, 90.00 |
Refinement procedure
| Resolution | 24.880 - 1.500 |
| R-factor | 0.2084 |
| Rwork | 0.207 |
| R-free | 0.24040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xt2 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.390 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmeas | 0.072 | 0.947 |
| Rpim | 0.038 | 0.555 |
| Number of reflections | 37270 | 3317 |
| <I/σ(I)> | 29.3 | 1.1 |
| Completeness [%] | 98.0 | 88.3 |
| Redundancy | 3.5 | 2.4 |
| CC(1/2) | 0.482 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 293 | Bis-Tris, pH5.4 and 16% PEG3350, Seeding with crushed crystals right after setup |
