6CZX
Crystal structure of Arabidopsis thaliana phosphoserine aminotransferase isoform 1 (AtPSAT1) in complex with PLP internal aldimine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.221, 105.929, 186.886 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 76.780 - 1.570 |
| R-factor | 0.15755 |
| Rwork | 0.157 |
| R-free | 0.17524 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xk1 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.541 |
| Data reduction software | XDS (May 1, 2016) |
| Data scaling software | XDS (May 1, 2016) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | REFMAC (5.8.0218) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.780 | 1.660 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmerge | 0.089 | 0.965 |
| Number of reflections | 230909 | 36591 |
| <I/σ(I)> | 13.33 | 1.85 |
| Completeness [%] | 99.2 | 98.1 |
| Redundancy | 7.4 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 292 | 0.2 M lithium sulfate, 17% PEG 3350 and 0.1 M Tris at pH 8.5 |
