6CZQ
A V-to-F substitution in SK2 channels causes Ca2+ hypersensitivity and improves locomotion in a C. elegans ALS model
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | LIQUID ANODE |
| Source details | BRUKER METALJET |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-08-04 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.3148 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 76.273, 66.726, 64.676 |
| Unit cell angles | 90.00, 92.19, 90.00 |
Refinement procedure
| Resolution | 26.866 - 2.200 |
| R-factor | 0.2007 |
| Rwork | 0.198 |
| R-free | 0.24930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.855 |
| Data reduction software | XPREP |
| Data scaling software | APEX 2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.866 | 2.279 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 16535 | 1618 |
| <I/σ(I)> | 14.44 | |
| Completeness [%] | 99.8 | 99.81 |
| Redundancy | 6.71 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293.15 | 0.1 M Sodium citrate tribasic dihydrate 0.5 M Ammonium sulfate 1.5 M Lithium sulfate monohydrate |
