6CZK

Crystal structure of wild-type human pro-cathepsin H

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-28
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 31 2 1
Unit cell lengths	97.416, 97.416, 107.530
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.368 - 2.001
R-factor	0.172
Rwork	0.171
R-free	0.19960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8pch
RMSD bond length	0.008
RMSD bond angle	0.922
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.077	0.510
Rmeas	0.087	0.553
Rpim	0.032	0.202
Number of reflections	40304	1973
<I/σ(I)>	13	6.1
Completeness [%]	100.0	100
Redundancy	7.4	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	293	0.1 M sodium phosphate dibasic, citric acid, pH 4.2, 2.0 M ammonium sulfate