6CZ1
Crystal structure of ATPase domain of Human GRP78 bound to Ver155008
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-31 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.280, 74.920, 86.200 |
| Unit cell angles | 90.00, 98.52, 90.00 |
Refinement procedure
| Resolution | 24.600 - 1.680 |
| R-factor | 0.189 |
| Rwork | 0.187 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5evz |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.21) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.263 | 1.720 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmeas | 0.073 | |
| Rpim | 0.032 | |
| Number of reflections | 79224 | 5822 |
| <I/σ(I)> | 17.5 | |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 24-26% PEG3350, 0.1 M Tris-HCl, 0.2 M sodium chloride |
