6CYO
Crystal structure of human UBE2A (RAD6A)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE W01B-MX2 |
Synchrotron site | LNLS |
Beamline | W01B-MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-04 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.45863 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.497, 51.350, 88.663 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.657 - 1.850 |
R-factor | 0.2045 |
Rwork | 0.204 |
R-free | 0.21400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2yb6 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.21) |
Phasing software | MOLREP |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.440 | 44.440 | 1.890 |
High resolution limit [Å] | 1.850 | 9.060 | 1.850 |
Rmerge | 0.064 | 0.040 | 1.692 |
Rmeas | 0.067 | 0.042 | 1.802 |
Rpim | 0.019 | 0.013 | 0.601 |
Number of reflections | 15168 | 164 | 910 |
<I/σ(I)> | 18.3 | ||
Completeness [%] | 99.4 | 99.8 | 98.3 |
Redundancy | 11.4 | 9.6 | 8.7 |
CC(1/2) | 0.999 | 0.998 | 0.602 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 50 mM di-Sodium succinate pH 7.0 and 12-14% PEG 3350 |