6CYN

CTX-M-14 N106S/D240G mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.999989
Spacegroup name	P 32
Unit cell lengths	83.238, 83.238, 231.982
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.420 - 1.600
R-factor	0.169
Rwork	0.168
R-free	0.20400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ylt
RMSD bond length	0.006
RMSD bond angle	0.810
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((DEV_3071: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.830
High resolution limit [Å]	1.600	1.800
Rmerge	0.119	0.662
Number of reflections	235349
<I/σ(I)>	14.2
Completeness [%]	99.4
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	296	25% PEG 1500 (w/v), 0.1 M PCB buffer, pH 6.0