6CYK
CTX-M-14 N106S mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-07-02 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.210, 107.160, 47.800 |
| Unit cell angles | 90.00, 101.72, 90.00 |
Refinement procedure
| Resolution | 22.920 - 1.700 |
| R-factor | 0.1529 |
| Rwork | 0.151 |
| R-free | 0.18520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pm5 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.853 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.400 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.091 | 0.516 |
| Number of reflections | 96247 | |
| <I/σ(I)> | 8.8 | |
| Completeness [%] | 99.6 | |
| Redundancy | 3.6 | |
| CC(1/2) | 0.993 | 0.721 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 296 | 0.2 M calcium acetate hydrate, 20% (w/v) PEG 3350, pH 7.5 |
