6CY7

Human Stimulator of Interferon Genes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 41 21 2
Unit cell lengths	111.457, 111.457, 35.424
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.010 - 2.200
R-factor	0.2052
Rwork	0.202
R-free	0.25753
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4loh
RMSD bond length	0.018
RMSD bond angle	1.935
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.000	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.062	0.657
Rmeas		0.726
Rpim	0.029	0.305
Number of reflections	11912	1696
<I/σ(I)>	14.2	2.6
Completeness [%]	99.9	100
Redundancy	5.7	5.5
CC(1/2)	0.999	0.759

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	285	ammonium citrate tribasic, imidazole, PEG5000 MME