6CY2

RNA octamer containing 2'-OMe, 4'Calpha-OMe U.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-17
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9183
Spacegroup name	P 43 21 2
Unit cell lengths	31.500, 31.500, 85.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.400 - 1.400
R-factor	0.185
Rwork	0.184
R-free	0.20510
Structure solution method	SAD
Data reduction software	xia2
Data scaling software	xia2
Phasing software	SHELXDE
Refinement software	PHENIX (1.13_2992)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.400	1.450
High resolution limit [Å]	1.400	1.400
Rpim	0.030	0.684
Number of reflections	16014
<I/σ(I)>	11
Completeness [%]	98.7
Redundancy	13.5
CC(1/2)	0.997	0.529

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	1 mM RNA; 40 mM sodium cacodylate, 80 mM potassium chloride, 20 mM magnesium chloride, 12 mM spermine. Reservoir 40% MPD