6CY0

RNA octamer containing 2'-F, 4'-Cbeta-OMe U.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-11
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9184
Spacegroup name	P 21 21 21
Unit cell lengths	47.012, 47.631, 100.744
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.602 - 2.398
R-factor	0.2043
Rwork	0.202
R-free	0.24230
Structure solution method	SAD
Data reduction software	xia2
Data scaling software	xia2
Phasing software	SHELXDE
Refinement software	PHENIX (1.13_2992)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.602	2.490
High resolution limit [Å]	2.398	2.398
Rmerge	0.198	0.433
Rpim	0.058	0.129
Number of reflections	9164	907
<I/σ(I)>	11
Completeness [%]	97.4
Redundancy	21.6
CC(1/2)	0.991	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	0.5 mM RNA; 100 mM MES, 2.5 M ammonium sulfate, 10 mM magnesium chloride,