6CXB

Structure of N-truncated R1-type pyocin tail fiber at 1.7 angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	120
Detector technology	PIXEL
Collection date	2013-12-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	I 21 3
Unit cell lengths	102.179, 102.179, 102.179
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.714 - 1.701
R-factor	0.1611
Rwork	0.159
R-free	0.17660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ct8
RMSD bond length	0.005
RMSD bond angle	0.788
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (2.6.0)
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.730
High resolution limit [Å]	1.700	4.610	1.700
Rmerge	0.074	0.026	0.919
Rmeas	0.077	0.027
Rpim	0.025	0.008	0.536
Number of reflections	19654	1053	981
<I/σ(I)>	11.8
Completeness [%]	100.0	100	99.8
Redundancy	9.4	10.5	3.6
CC(1/2)		0.999	0.520

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	Crystals were grown at a 1:1 protein (5 mg/mL) to precipitant (100mM Bicine pH 9.5, 20% PEG2k, 1mM CuSO4) ratio