6CX4

V180A Mutant of Yeast PCNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-15
Detector	NOIR-1
Wavelength(s)	0.97
Spacegroup name	P 21 3
Unit cell lengths	122.555, 122.555, 122.555
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.510 - 3.081
R-factor	0.2473
Rwork	0.246
R-free	0.26660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1plq
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	24.510	24.510	3.190
High resolution limit [Å]	3.080	6.600	3.080
Rmerge	0.108	0.047	0.720
Rmeas	0.116	0.051	0.765
Total number of observations	99880
Number of reflections	11585	1228	1150
<I/σ(I)>	9.3	28.6	2
Completeness [%]	100.0	100	100
Redundancy	8.56	7.83	8.81

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290.15	2.2M Ammonium Sulfate 0.2M Potassium Chloride 20% Glycerol