6CX0
Structure of AtTPC1 D376A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 88.470, 154.420, 218.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.000 - 3.501 |
| R-factor | 0.3199 |
| Rwork | 0.318 |
| R-free | 0.35190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dqq |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.687 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 4.000 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmeas | 0.239 | |
| Number of reflections | 19231 | 6273 |
| <I/σ(I)> | 8.08 | |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 13.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.3 | 292 | Sitting drop 2microliter drops crystchem 0.1 M glycine, pH 9.3, 50 mM potassium chloride, 1 mM calcium chloride, 35% PEG300 |
