6CW2
Crystal structure of a yeast SAGA transcriptional coactivator Ada2/Gcn5 HAT subcomplex, crystal form 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-26 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 191.816, 191.816, 92.674 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 95.908 - 2.670 |
| R-factor | 0.2517 |
| Rwork | 0.250 |
| R-free | 0.28380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.733 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 166.120 | 2.750 |
| High resolution limit [Å] | 2.670 | 2.670 |
| Rpim | 0.045 | 0.947 |
| Number of reflections | 55876 | 4546 |
| <I/σ(I)> | 15.4 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 20.1 | 19.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 8.5 | 294 | 10 mM Tris-Cl pH 8.5, 200 mM Li2SO4, 25% w/v PEG3350 |
