6CW0
Crystal structure of Cryptosporidium parvum bromodomain cgd2_2690
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97887 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.543, 71.422, 98.274 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.010 - 1.380 |
| R-factor | 0.177 |
| Rwork | 0.176 |
| R-free | 0.19990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4z93 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.323 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0218) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.400 |
| High resolution limit [Å] | 1.380 | 3.750 | 1.380 |
| Rmerge | 0.085 | 0.059 | 0.969 |
| Rmeas | 0.091 | 0.063 | 1.042 |
| Rpim | 0.031 | 0.022 | 0.379 |
| Total number of observations | 403015 | ||
| Number of reflections | 49181 | 2691 | 2428 |
| <I/σ(I)> | 6.7 | ||
| Completeness [%] | 100.0 | 99.9 | 100 |
| Redundancy | 8.2 | 7.8 | 7.4 |
| CC(1/2) | 0.997 | 0.699 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 2.5M NH4SO4 and 0.1M BTP 7.0 |
