6CUQ

Crystal structure of Macrophage migration inhibitory factor-like protein (EhMIF) from Entamoeba histolytica

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2018-03-01
Detector	RAYONIX MX-300
Wavelength(s)	0.97872
Spacegroup name	C 2 2 21
Unit cell lengths	77.610, 100.060, 98.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.376 - 2.450
R-factor	0.1822
Rwork	0.177
R-free	0.22530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3t5s selected by MorDa
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MoRDa
Refinement software	PHENIX ((dev_3063))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.376	38.376	2.510
High resolution limit [Å]	2.450	10.960	2.450
Rmerge	0.032	0.023	0.547
Rmeas	0.035	0.026	0.587
Number of reflections	14405	183	1048
<I/σ(I)>	32.96	70.09	4.2
Completeness [%]	99.9	94.8	99.9
Redundancy	7.349	5.475	7.498
CC(1/2)	1.000	0.999	0.954

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	290	Microlytics MCSG1 screen, condition G9: 20% PEG 3350, 200mM sodium formate: EnhiA.00834.a.B1.PS38385 at 22.83mg/ml: cryo: 20% EG: tray 297191g9: puck gmt2-2