6CT8

Selenomethionine structure of N-truncated R2-type pyocin tail fiber at 2.6 angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	120
Detector technology	PIXEL
Collection date	2013-12-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.980
Spacegroup name	P 63 2 2
Unit cell lengths	59.930, 59.930, 396.633
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.983 - 2.620
R-factor	0.1849
Rwork	0.179
R-free	0.23420
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.851
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (2.6.0)
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.670
High resolution limit [Å]	2.620	7.110	2.620
Rmerge	0.060	0.041	0.117
Rmeas	0.064	0.044	0.128
Rpim	0.021	0.015	0.049
Total number of observations	103793
Number of reflections	13424	860	633
<I/σ(I)>	17.8
Completeness [%]	96.5	97.6	97.1
Redundancy	7.7	7.7	6
CC(1/2)		0.999	0.993

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	Crystals were obtained at a 1:1 protein (6mg/mL) to precipitant (1M Na Acetate, 100mM CAPS, 100 mM LiOAc)