6CR6
Ternary complex crystal structure of DNA polymerase Beta with a dideoxy terminated primer with CH-CH3, beta, gamma dATP analogue
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-20 |
Detector | RIGAKU SATURN 92 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.141, 79.236, 55.539 |
Unit cell angles | 90.00, 106.69, 90.00 |
Refinement procedure
Resolution | 20.846 - 2.097 |
R-factor | 0.2228 |
Rwork | 0.216 |
R-free | 0.29300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fms |
RMSD bond length | 0.009 |
RMSD bond angle | 1.036 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
Rmerge | 0.073 | 0.033 | 0.609 |
Total number of observations | 74235 | ||
Number of reflections | 22923 | 2383 | 2014 |
<I/σ(I)> | 15.3 | ||
Completeness [%] | 95.9 | 97.2 | 85.4 |
Redundancy | 3.2 | 3.4 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 16-18% PEG 3350 |