6CQM

Crystal Structure of mitochondrial single-stranded DNA binding proteins from S. cerevisiae, Rim1 (Form2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2016-01-27
Detector	RDI CMOS_8M
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	82.470, 103.540, 114.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.128 - 3.000
R-factor	0.2407
Rwork	0.238
R-free	0.29410
Structure solution method	SAD
RMSD bond length	0.001
RMSD bond angle	0.372
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.140	3.110
High resolution limit [Å]	3.000	3.000
Rmerge	0.035	0.231
Rpim	0.035	0.231
Number of reflections	24765	1977
<I/σ(I)>	19
Completeness [%]	99.8
Redundancy	13.9
CC(1/2)	0.998	0.867

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.2	295	0.2 M MgCl2 and 20% (w/v) PEG 3350