6CQF

Crystal structure of HPK1 in complex an inhibitor G1858

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	93
Detector technology	CCD
Collection date	2014-03-12
Detector	RAYONIX MX-300
Wavelength(s)	0.97872
Spacegroup name	C 2 2 21
Unit cell lengths	91.868, 98.803, 77.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.639 - 2.246
R-factor	0.2101
Rwork	0.208
R-free	0.25510
RMSD bond length	0.005
RMSD bond angle	0.954
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.330
High resolution limit [Å]	2.250	2.250
Number of reflections	16438
<I/σ(I)>	27.4
Completeness [%]	96.4
Redundancy	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	292	0.1 M Tris-HCl, pH 8.5, 0.25 M sodium tartrate, 12% PEG8000