6CPO
Crystal structure of DR15 presenting the RQ13 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-09 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.740, 73.500, 94.940 |
| Unit cell angles | 90.00, 106.95, 90.00 |
Refinement procedure
| Resolution | 47.080 - 2.400 |
| R-factor | 0.205 |
| Rwork | 0.203 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mdo |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.990 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.200 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rpim | 0.076 | 0.408 |
| Number of reflections | 34562 | 3604 |
| <I/σ(I)> | 9 | |
| Completeness [%] | 100.0 | |
| Redundancy | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | PBS pH 7.5, Na acetate, PEG 4000 |
