6CPE
Structure of apo, dephosphorylated Aurora A (122-403) in an active conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00001 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 80.550, 80.550, 169.790 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 64.520 - 2.450 |
| R-factor | 0.2183 |
| Rwork | 0.214 |
| R-free | 0.25360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mq4 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.602 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.900 | 2.550 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.068 | 1.202 |
| Number of reflections | 12688 | 8694 |
| <I/σ(I)> | 15 | |
| Completeness [%] | 99.9 | |
| Redundancy | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 291.15 | A 1:1 ratio protein:mother liquor was obtained by mixing 0.5 uL Aurora A (300 uM; 10 mg/mL) in 50 mM HEPES, pH 7.3, 500 mM ammonium acetate, 1 mM MgCl2, 5 mM TCEP) with 0.5 uL of 0.15 M Tris-HCl, pH 7.5, 0.15 M ammonium sulfate, 35% (w/v) PEG-3350 |
