6CP8
Contact-dependent growth inhibition toxin-immunity protein complex from from E. coli 3006
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.707, 106.534, 72.654 |
Unit cell angles | 90.00, 101.01, 90.00 |
Refinement procedure
Resolution | 29.632 - 2.201 |
R-factor | 0.1774 |
Rwork | 0.176 |
R-free | 0.21810 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.823 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.127 | 0.749 |
Number of reflections | 38085 | 1879 |
<I/σ(I)> | 11.28 | 1.96 |
Completeness [%] | 99.9 | 100 |
Redundancy | 4.2 | 3.8 |
CC(1/2) | 0.664 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.1 M HEPES/Na pH 7.0, 18% PEG12K |