6CNW

STRUCTURE OF HUMANIZED SINGLE DOMAIN ANTIBODY SD84

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-20
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0
Spacegroup name	P 41
Unit cell lengths	54.570, 54.570, 35.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.404 - 0.920
R-factor	0.1263
Rwork	0.126
R-free	0.13940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.009
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.270	0.970
High resolution limit [Å]	0.920	0.920
Number of reflections	63610	3146
<I/σ(I)>	20.39	2.17
Completeness [%]	86.2	29.3
Redundancy	5.76	2.46
CC(1/2)	1.000	0.780

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	295	2.4 M (NH4)2SO4, 0.1 M sodium acetate, 5% PEG 400