6CNP

Crystal structure of MBD2 complex with methylated CpG island

Replaces:  6C2K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-18
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97947
Spacegroup name	P 1
Unit cell lengths	40.960, 41.320, 58.670
Unit cell angles	101.57, 99.46, 100.54

Refinement procedure

Resolution	39.457 - 2.100
R-factor	0.1906
Rwork	0.189
R-free	0.21770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdb entries 2KY8 3qmg
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.460	39.460	2.160
High resolution limit [Å]	2.100	8.910	2.100
Rmerge	0.044	0.032	0.970
Rmeas	0.051	0.038	1.122
Rpim	0.026	0.020	0.563
Total number of observations	80533
Number of reflections	20704		1697
<I/σ(I)>	15.3
Completeness [%]	97.8	95	97.2
Redundancy	3.9	3.5	3.9
CC(1/2)	0.998	0.997	0.685

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	8% PEG550MME, 8% PEG20000, 0.2M calcium acetate