6CN0

2.95 Angstrom Crystal Structure of 16S rRNA Methylase from Proteus mirabilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-14
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97856
Spacegroup name	P 61
Unit cell lengths	158.318, 158.318, 122.892
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.980 - 2.950
R-factor	0.22014
Rwork	0.218
R-free	0.26286
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lcu
RMSD bond length	0.007
RMSD bond angle	1.298
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.000
High resolution limit [Å]	2.950	2.950
Rmerge	0.053	0.874
Rmeas	0.060	0.986
Rpim	0.027	0.446
Number of reflections	36800	1815
<I/σ(I)>	25.7	2
Completeness [%]	99.9	100
Redundancy	4.7	4.7
CC(1/2)		0.612

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	292	Protein: 10.0 mg/ml, 0.3M Sodium chloride, 2mM Magnesium chloride, 0.01M HEPES (pH 7.5), Screen: 2M Ammonium Sulfate, 0.1M Sodium citrate (pH 5.6), 1% Isopropanol, Cryo: paratone