6CMJ
Human CAMKK2 with GSK650393
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-08 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 89.841, 89.841, 181.385 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.970 - 2.400 |
| R-factor | 0.1845 |
| Rwork | 0.182 |
| R-free | 0.21920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.521 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.480 |
| High resolution limit [Å] | 2.390 | 5.150 | 2.390 |
| Rmerge | 0.061 | 0.035 | 0.311 |
| Number of reflections | 33208 | 3652 | 2484 |
| <I/σ(I)> | 12.6 | ||
| Completeness [%] | 96.2 | 98.8 | 73.2 |
| Redundancy | 8.1 | 8.4 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 4 M sodium formate, 3% PEG8000, 20 mM ammonium sulfate |
