6CMC
Barium sites in the structure of a desensitized acid sensing ion channel
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-08-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.93725 |
| Spacegroup name | H 3 |
| Unit cell lengths | 133.938, 133.938, 122.671 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.140 - 3.671 |
| R-factor | 0.2842 |
| Rwork | 0.283 |
| R-free | 0.29950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nyk |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.978 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_2597: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.140 | 3.800 |
| High resolution limit [Å] | 3.670 | 3.670 |
| Number of reflections | 8949 | |
| <I/σ(I)> | 6.78 | |
| Completeness [%] | 99.6 | |
| Redundancy | 8.12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 293.15 | 100 mM HEPES pH 6.9, 150 mM sodium formate, 18% (w/v) PEG 3,350. Crystal soaked in 50 mM BaCl2 prior to freezing |
