6CLW
Crystal structure of TnmH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2017-09-27 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 110.860, 110.860, 113.310 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 56.660 - 2.740 |
R-factor | 0.20501 |
Rwork | 0.202 |
R-free | 0.25436 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3i53 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.385 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.660 | 2.890 |
High resolution limit [Å] | 2.740 | 2.740 |
Number of reflections | 21574 | |
<I/σ(I)> | 10 | |
Completeness [%] | 99.9 | |
Redundancy | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2 M L-Proline, 0.1 M HEPES, pH 7.5, 10% w/v Polyethylene glycol 3,350 |