6CL4

LipC12 - Lipase from metagenomics

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-07-29
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 41 2 2
Unit cell lengths	58.500, 58.500, 192.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.175 - 2.640
R-factor	0.195
Rwork	0.192
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ex9
RMSD bond length	0.002
RMSD bond angle	0.447
Data reduction software	XDS (VERSION December 28, 2009)
Data scaling software	XSCALE (VERSION January 30, 2009)
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.500	2.720
High resolution limit [Å]	2.640	2.640
Rmeas	0.140	0.641
Number of reflections	10489	851
<I/σ(I)>	18.91	4.29
Completeness [%]	95.5	97.7
Redundancy	8.82	8.38

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	2.0 M sodium formate and 0.1 M bis-tris propane, pH 7.0 using a protein concentration of 10 mg / mL. Crystals grown for ten weeks