6CKI

Co-crystal structure of MNK2 in Complex With Inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.987
Spacegroup name	P 32 2 1
Unit cell lengths	104.277, 104.277, 71.359
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 - 2.950
R-factor	0.19317
Rwork	0.190
R-free	0.25739
RMSD bond length	0.013
RMSD bond angle	1.836
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall
Low resolution limit [Å]	56.700
High resolution limit [Å]	2.950
Number of reflections	9676
<I/σ(I)>	2
Completeness [%]	98.0
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	300.15	20-30% (w/v) polyacrylic acid 5100, 1-5% PEG 400, and 50 mM Hepes (pH 7.5)