6CK9

Crystal Structure of HIV-1 ConC_Base0 Prefusion Env Trimer in Complex with Human Antibody Fragment 3H109L and 35O22 variants at 3.5 Angstrom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	298
Detector technology	CCD
Collection date	2017-08-22
Detector	RAYONIX MX300-HS
Wavelength(s)	1
Spacegroup name	P 63
Unit cell lengths	131.900, 131.900, 314.860
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.174 - 2.714
R-factor	0.2504
Rwork	0.248
R-free	0.28760
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.760
High resolution limit [Å]	2.710	7.350	2.710
Rmerge	0.108	0.057	1.693
Rmeas	0.126	0.067	2.035
Rpim	0.064	0.034	1.095
Total number of observations	153622
Number of reflections	29641	4222	290
<I/σ(I)>	5.7
Completeness [%]	53.5	99.2	6.9
Redundancy	3.4	3.7	2.3
CC(1/2)		0.990	0.221

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	75mM HEPES pH7.5; 2% PEG 8,000 and 10% PEG 400