6CJW

Crystal Structure of Mnk2-D228G in Complex With Inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.987
Spacegroup name	P 32 2 1
Unit cell lengths	105.645, 105.645, 73.537
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 - 3.380
R-factor	0.1803
Rwork	0.177
R-free	0.24814
RMSD bond length	0.013
RMSD bond angle	2.005
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.700	3.200
High resolution limit [Å]	3.000	3.000
Number of reflections	9662
<I/σ(I)>	4
Completeness [%]	98.0
Redundancy	8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	300	20-30% (w/v) polyacrylic acid 5100, 4% PEG 400, 50 mM Hepes, pH7.5.