6CJC

CRYSTAL STRUCTURE OF A FC FRAGMENT LALA MUTANT (L234A, L235A) OF HUMAN IGG1 (CRYSTAL FORM 3)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-31
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	48.962, 75.188, 148.295
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.530 - 2.575
R-factor	0.195
Rwork	0.193
R-free	0.24060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ave
RMSD bond length	0.005
RMSD bond angle	1.008
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.575	2.575
Rmerge	0.232
Rpim	0.100
Number of reflections	17739	845
<I/σ(I)>	15.3	1.3
Completeness [%]	99.7	100
Redundancy	6.3
CC(1/2)		0.274

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	20% PEG MME 5,000 0.1 M Bis-Tris. pH 6.5