6CI4

Crystal structure of the formyltransferase PseJ from Anoxybacillus kamchatkensis soaked with UDP-4-amino-4,6-dideoxy-L-AltNAc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-12
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	96.425, 73.780, 41.805
Unit cell angles	90.00, 103.47, 90.00

Refinement procedure

Resolution	46.885 - 1.824
R-factor	0.188269814514
Rwork	0.186
R-free	0.21113
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ci2
RMSD bond length	0.011
RMSD bond angle	1.217
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.890	1.850
High resolution limit [Å]	1.820	1.820
Rpim		0.398
Number of reflections	23891	1025
<I/σ(I)>	12.1
Completeness [%]	93.9
Redundancy	2.8
CC(1/2)		0.691

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	295	0.2 M ammonium sulfate, 0.1 M MES pH 6.5, 28.4% PEG5000 MME