6CHG

Crystal structure of the yeast COMPASS catalytic module

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-11-16
Detector	ADSC QUANTUM 270
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	163.839, 138.319, 136.133
Unit cell angles	90.00, 112.41, 90.00

Refinement procedure

Resolution	44.607 - 2.985
R-factor	0.2301
Rwork	0.228
R-free	0.27080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5f6k 2h14
RMSD bond length	0.003
RMSD bond angle	0.613
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.050
High resolution limit [Å]	2.985	2.985
Rmerge	0.151
Rmeas	0.165
Rpim	0.063	0.474
Number of reflections	54818	2416
<I/σ(I)>	14.73	1.175
Completeness [%]	96.9	86.5
Redundancy	6.6	5
CC(1/2)		0.569

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	Sodium Tartrate, PEG20000, ATP