6CG8

Structure of C. crescentus GapR-DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	273
Detector technology	PIXEL
Collection date	2017-12-16
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	95.913, 35.579, 115.559
Unit cell angles	90.00, 90.18, 90.00

Refinement procedure

Resolution	57.779 - 2.299
R-factor	0.251
Rwork	0.249
R-free	0.27710
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.690
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AutoSol
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.800	2.340
High resolution limit [Å]	2.299	2.299
Rpim	0.071	0.267
Number of reflections	32492	3035
<I/σ(I)>	7.6
Completeness [%]	99.0	98
Redundancy	3.5	3.5
CC(1/2)	0.977	0.956

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG 400, CaCl2, HEPES