6CFY

Bosea sp Root 381 apo GapR structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	273
Detector technology	PIXEL
Collection date	2017-12-16
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	P 41 2 2
Unit cell lengths	39.589, 39.589, 205.125
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.800 - 2.400
R-factor	0.2344
Rwork	0.231
R-free	0.26590
Structure solution method	SAD
RMSD bond length	0.018
RMSD bond angle	1.675
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AutoSol
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.870	2.530
High resolution limit [Å]	2.400	2.400
Rmeas	0.122
Rpim	0.041
Number of reflections	12187	1213
<I/σ(I)>	11.2
Completeness [%]	100.0	99.9
Redundancy	19.1
CC(1/2)	0.997	0.319

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	15% MPD, 10 mM MgCl2, HEPES (7.5) 0.001 M spermine