6CFX
Bosea sp GapR solved in the presence of DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 273 |
| Detector technology | PIXEL |
| Collection date | 2017-03-23 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.328, 44.483, 107.051 |
| Unit cell angles | 90.00, 101.82, 90.00 |
Refinement procedure
| Resolution | 50.240 - 2.000 |
| R-factor | 0.2001 |
| Rwork | 0.199 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6cg8 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.504 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.240 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmeas | 0.072 | 0.017 |
| Rpim | 0.042 | 0.011 |
| Number of reflections | 29676 | 1921 |
| <I/σ(I)> | 10.1 | 3.9 |
| Completeness [%] | 92.0 | 69.1 |
| Redundancy | 2.5 | 2 |
| CC(1/2) | 0.994 | 0.975 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | ammonium sulphate, MES buffer pH 6.5 |
