6CFT

Structure of Human alpha-Phosphomannomutase 1 containing mutations R180T and R183I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-08-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9791
Spacegroup name	P 43 21 2
Unit cell lengths	51.350, 51.350, 214.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.938 - 2.430
R-factor	0.2153
Rwork	0.208
R-free	0.27570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fuc
RMSD bond length	0.008
RMSD bond angle	1.143
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.940	2.540
High resolution limit [Å]	2.430	2.430
Number of reflections	11589
<I/σ(I)>	21.22
Completeness [%]	99.8
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290	20% polyethylene glycol 3350, 0.15 M DL-malate, pH 7.0, 50 mM MgCl2, 0.05 M L-lysine and 8 mM beta-mercaptoethanol