6CF9
Crystal structure of soluble lytic transglycosylase Cj0843 of Campylobacter jejuni at 2.3A resolution in I23 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-27 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97940 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 178.674, 178.674, 178.674 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 126.340 - 2.290 |
| R-factor | 0.2014 |
| Rwork | 0.200 |
| R-free | 0.23210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6cf8 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.286 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 126.340 | 2.350 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Number of reflections | 42902 | |
| <I/σ(I)> | 21.5 | |
| Completeness [%] | 99.6 | |
| Redundancy | 40.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 0.2 M Ammonium acetate, 0.1 M sodium acetate pH 4.6, and 30% (w/v) PEG 4000 |
