6CF8
Crystal structure of Cj0843 lytic transglycosylase of Campylobacter jejuni at 1.87A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-29 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 74.640, 74.640, 194.086 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 64.700 - 1.870 |
| R-factor | 0.1934 |
| Rwork | 0.191 |
| R-free | 0.24170 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.292 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.700 | 1.920 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Number of reflections | 51659 | |
| <I/σ(I)> | 16.3 | |
| Completeness [%] | 98.3 | |
| Redundancy | 19 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 0.2 M lithium sulfate, 0.1 M Tris pH 8.0, and 39% PEG 400 |
