6CF6

RNF146 TBM-Tankyrase ARC2-3 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-31
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.99995
Spacegroup name	C 1 2 1
Unit cell lengths	134.019, 103.284, 75.163
Unit cell angles	90.00, 106.93, 90.00

Refinement procedure

Resolution	50.000 - 1.930
R-factor	0.197
Rwork	0.195
R-free	0.22790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3utm
RMSD bond length	0.010
RMSD bond angle	1.340
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.990
High resolution limit [Å]	1.930	4.420	1.930
Rmerge	0.054	0.025	0.465
Rmeas	0.063	0.030	0.582
Rpim	0.033	0.015	0.344
Number of reflections	71212	6188	4526
<I/σ(I)>	11.8
Completeness [%]	97.2	99.8	74.3
Redundancy	3.6	3.7	2.3
CC(1/2)		0.999	0.775

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	30 mM sodium citrate pH 5.6, 60 mM ammonium acetate, 27% 2-methyl-2,4-pentanediol