6CEZ
Crystal Structure of Rabbit Anti-HIV-1 gp120 V2 Fab 16C2 in complex with V2 peptide ConB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-20 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.979460 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.145, 41.470, 79.893 |
| Unit cell angles | 90.00, 91.68, 90.00 |
Refinement procedure
| Resolution | 39.929 - 2.399 |
| R-factor | 0.2402 |
| Rwork | 0.237 |
| R-free | 0.29580 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.425 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.930 | 2.540 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmeas | 0.061 | 0.690 |
| Number of reflections | 32149 | 2679 |
| <I/σ(I)> | 20.2 | 2.16 |
| Completeness [%] | 99.8 | 99.4 |
| Redundancy | 3.71 | 3.73 |
| CC(1/2) | 0.999 | 0.842 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 23% polyethylene glycol 3350, 0.2 M LiCL, 0.1 M 2-ethanesulfonic acid (MES) pH 6.5 |
