6CEW

Segment AMMAAA from the low complexity domain of TDP-43, residues 321-326

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	9.520, 15.440, 44.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.050 - 1.200
R-factor	0.1989
Rwork	0.198
R-free	0.21840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	IDEAL BETA STRAND
RMSD bond length	0.012
RMSD bond angle	1.431
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.7.17)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	22.050	22.050	1.230
High resolution limit [Å]	1.200	5.370	1.200
Rmerge	0.181	0.031	0.683
Rmeas	0.214	0.038	0.800
Number of reflections	2209	40	145
<I/σ(I)>	3.16	4.48	1.43
Completeness [%]	96.0	100	99.3
Redundancy	3.507	2.6	3.497
CC(1/2)	0.995	1.000	0.692

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	298	1.3 M ammonium phosphate, 0.1 M sodium acetate pH 6.3