6CD2
Crystal structure of the PapC usher bound to the chaperone-adhesin PapD-PapG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 204.303, 300.710, 100.515 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.010 - 3.700 |
| R-factor | 0.28433 |
| Rwork | 0.282 |
| R-free | 0.32932 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vqi 2wmp 1j8s 3l48 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.260 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.830 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rmerge | 0.122 | |
| Rmeas | 0.134 | |
| Rpim | 0.054 | 0.448 |
| Number of reflections | 27152 | 3048 |
| <I/σ(I)> | 15.9 | 1.5 |
| Completeness [%] | 98.5 | 92.5 |
| Redundancy | 6 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 50 mM sodium citrate pH 5.0-6.0, 50 mM lithium sulfate, 50 mM sodium sulfate and 7-14% PEG 4000 |
